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AssigneesMedical Research Council, Cambridge Antibody Technology Limited
InventorsKaspar-Philipp Holliger, Andrew David Griffiths, Hendricus Renerus Jacobus Matheus Hoogenboom, Magnus Malmqvist, James David Marks, Brian Timothy McGuinness, Anthony Richard Pope, Terence Derek Prospero, Gregory Paul Winter
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Polypeptides comprising a first domain, which comprises a binding region of an immunoglobulin heavy chain variable region, and a second domain, which comprises a binding region of an immunoglobulin light chain variable region, the domains being linked but incapable of associating with each other to form an antigen binding site, associate to form antigen binding multimers, such as dimers, which may be multivalent or have multispecificity. The domains may be linked by a short peptide linker or may be joined directly together. Bispecific dimers may have longer linkers. Methods of preparation of the polypeptides and multimers and diverse repertoires thereof, and their display on the surface of bacteriophage for easy selection of binders of interest, are disclosed, along with many utilities.
A dimer comprising:
A vector comprising a nucleotide sequence encoding a first polypeptide and a nucleotide sequence encoding a second polypeptide, each of the first and second encoded polypeptides comprising a first domain which comprises a binding region of an immunoglobulin heavy chain variable region, and a second domain which comprises a binding region of an immunoglobulin light chain variable region, the first domain of each polypeptide being linked to the second domain of that polypeptide directly to each other by an amide bond or by a polypeptide linker of from 1 to 5 amino acids;
A vector comprising:
A dimer comprising:
A vector comprising a nucleotide sequence encoding a first polypeptide and a nucleotide sequence encoding a second polypeptide each of the first and second encoded polypeptides comprising a first domain which comprises a binding region of an immunoglobulin heavy chain variable region, and a second domain which comprises a binding region of an immunoglobulin light chain variable region, the first domain of each polypeptide being linked to the second domain of that polypeptide directly to each other by an amide bond;
A dimer comprising:
A vector comprising a nucleotide sequence encoding a first polypeptide and a nucleotide sequence encoding a second polypeptide each of the first and second encoded polypeptides comprising a first domain which comprises a binding region of an immunoglobulin heavy chain variable region, and a second domain which comprises a binding region of an immunoglobulin light chain variable region, the first domain of each polypeptide being linked to the second domain of that polypeptide directly to each other by a polypeptide linker of 5 amino acids;
A dimer comprising:
A vector comprising a nucleotide sequence encoding a first polypeptide and a nucleotide sequence encoding a second polypeptide each of the first and second encoded polypeptides comprising a first domain which comprises a binding region of an immunoglobulin heavy chain variable region, and a second domain which comprises a binding region of an immunoglobulin light chain variable region, the first domain of each polypeptide being linked to the second domain of that polypeptide directly to each other by a polypeptide linker of 4 amino acids;
A dimer comprising:
A dimer comprising:
A dimer comprising:
A vector comprising a nucleotide sequence encoding a first polypeptide and a nucleotide sequence encoding a second polypeptide each of the first and second encoded polypeptides comprising a first domain which comprises a binding region of an immunoglobulin heavy chain variable region, and a second domain which comprises a binding region of an immunoglobulin light chain variable region, the first domain of each polypeptide being linked to the second domain of that polypeptide directly to each other by a polypeptide linker of from 1 to 3 amino acids;
Dependencies are inferred from the claim text.