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US 5,834,273 AGranted
Heat-stable and water soluble modified enzymes
AssigneeWako Pure Chemical Industries, Ltd.
InventorsMasayuki Futatsugi, Kenji Gushi
Life of this patent
Estimating the term…
Abstract
A modified enzyme obtained by modifying an enzyme such as a hydrogen peroxide-generating enzyme, ascorbate oxidase, etc. with a polysaccharide, polyamino acid or synthetic polymer having a plurality of carboxyl groups via a crosslinking agent capable of binding both carboxyl group and amino group is remarkably improved in resistance to heat, proteases, etc. and in storage stability in an aqueous solution.
Claims8
A heat stable and Water soluble modified enzyme consisting essentially of
- uricase modified by reacting 0.1 to 10 weight parts of uricase with 1 weight part of a polyamino acid containing 25% or more of glutamic acid or aspartic acid residues through forming carboxylic acid amide linkage between the amino groups of uricase and the carboxylic acid groups of the polyamino acid.
A heat stable and water soluble modified enzyme
- selected from the group consisting of choline oxidase, glucose oxidase, xanthine oxidase, oxalate oxidase, sarcosine oxidase, ascorbate oxidase, urease, catalase and glycerokinase, said enzyme modified by reacting 0.1 to 10 weight parts of the enzyme with 1 weight part of a) a polysaccharide having a molecular weight of 4,000 to 10,000,000 and containing 0.1 to 5 carboxyl groups per sugar unit of the polysaccharide, or b) a polyamino acid containing 25% or more of glutamic acid or aspartic acid residues through forming carboxylic acid amide linkage between the amino groups of the enzyme and the carboxcylic acid groups of the polysaccharide or the polyamino acid.
Dependencies are inferred from the claim text.