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US 5,834,251 AGranted
Methods of modifying carbohydrate moieties
AssigneeAlko Group Ltd.
InventorsMarleen Maras, Roland Contreras
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Abstract
The invention is directed to methods of converting high mannose type glycosylation patterns to hybrid or complex type glycosylation patterns.
Claims20
A process for producing a hybrid glycoprotein, said process comprising:
- (a) reacting a high mannose type glycoprotein that has been produced in filamentous fungi with GlcNAc Tr I in the presence of a UDP-GlcNAc sugar nucleotide;
- (b) reacting the glycoprotein product of step (a) with .beta.-1,4 galactosyltransferase in the presence of a UDP-galactose sugar nucleotide;
- and
- (c) reacting the glycoprotein product of step (b) with a sialyltransferase in the presence of a CMP-sialic acid sugar nucleotide;
- wherein said filamentous fungus is a member of the group consisting of:
- Aspergillis and Trichoderma.
3 more elements
A process for producing a hybrid glycoprotein, said method comprising:
- (a) reacting a high mannose type glycoprotein expressed in yeast or a filamentous fungus with an .alpha.-1,2-mannosidase;
- (b) reacting the glycoprotein product of step (a) with GlcNAc Tr I in the presence of a UDP-GlcNAc sugar nucleotide;
- (c) reacting the glycoprotein product of step (b) with .beta.-1,4 galactosyltransferase in the presence of a UDP-galactose sugar nucleotide;
- and
- (d) reacting the glycoprotein product of step (c) with a sialyltransferase in the presence of a CMP-sialic acid sugar nucleotide;
- wherein said yeast or said filamentous fungus is selected from the group consisting of:
- Aspergillus, Trichoderma, Pichia pastoris, Hansenula polymorpha, Kluyveromyces lactis, Saccharomyces cerevisiae and Yarrowia lipolytica.
4 more elements
Dependencies are inferred from the claim text.