Method for detecting antipolymer antibodies and diagnosing silicone related disease (SRD) fibromyalgia and chronic fatigue syndrome (CFS)
AssigneeThe Administrators of the Tulane Educational Fund
InventorsRobert F. Garry, Scott A. Tenenbaum, Douglas R. Plymale
Life of this patent
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Abstract
The present invention provides for a method of detecting antipolymer antibodies, and a method for detecting silicone related disease, fibromyalgia, and chronic fatigue syndrome.
Claims10
A method of detecting antipolymer antibody, comprising the steps of:
- a) providing a sample to be tested for an antipolymer antibody;
- b) combining partially polymerized acrylamide with said sample for a time sufficient for an antipolymer antibody to react with said polymer, to form a complex;
- and
- c) reacting an indicator reagent with the material resulting from step b) to indicate the presence or absence of an antipolymer antibody in said sample.
A diagnostic kit for use in detecting antipolymer antibody, comprising:
- a polymer antigen comprising partially polymerized acrylamide attached to a support material;
- and
- an indicator reagent comprising a binding member that is specific for human antibody and conjugated to a detectable label.
A method to aid the diagnosis of silicone related disease (SRD) comprising the steps of:
- a) providing a sample to be tested for an antipolymer antibody;
- b) combining a polymer antigen comprising partially polymerized acrylamide with said sample for a time sufficient for an antipolymer antibody to react with said polymer antigen, to form a complex;
- c) reacting an indicator reagent with the material resulting from step b) to indicate the presence or absence of an antipolymer antibody in said sample;
- and
- d) identifying the presence or absence of reacted indicator reagent in the material resulting from step c);
- whereby the presence of reacted indicator reagent identifies silicone related disease in an individual providing said sample, with a clinical diagnosis of SRD.
A method to aid the diagnosis of fibromyalgia, comprising the steps of:
- a) providing a sample to be tested for an antipolymer antibody;
- b) combining a polymer antigen comprising partially polymerized acrylamide with said sample for a time sufficient for an antipolymer antibody to react with said polymer antigen, to form a complex;
- c) reacting an indicator reagent with the material resulting from step b) to indicate the presence or absence of an antipolymer antibody in said sample;
- and
- d) identifying the presence or absence of reacted indicator reagent in the material resulting from step c);
- whereby the presence of reacted indicator reagent identifies fibromyalgia in an individual providing said sample, with a clinical diagnosis of fibromyalgia.
A method to aid the diagnosis of chronic fatigue syndrome (CFS) comprising the steps of:
- a) providing a sample to be tested for an antipolymer antibody;
- b) combining a polymer antigen comprising partially polymerized acrylamide with said sample for a time sufficient for an antipolymer antibody to react with said polymer antigen, to form a complex;
- c) reacting an indicator reagent with the material resulting from step b), to indicate the presence or absence of an antipolymer antibody in said sample;
- and
- d) identifying the presence or absence of reacted indicator reagent in the material resulting from step c);
- whereby the presence of reacted indicator reagent identifies CFS in an individual providing said sample, with a clinical diagnosis of chronic fatigue.
Dependencies are inferred from the claim text.