Canarypox virus expressing cytokine and/or tumor-associated antigen DNA sequence

A recombinant poxvirus containing therein an exogenous DNA sequence encoding a cytokine, an exogenous DNA sequence encoding a tumor-associated antigen, or exogenous DNA sequences encoding a cytokine and a tumor associated antigen in a nonessential region of the poxvirus genome wherein the poxvirus expresses the exogenous DNA and the poxvirus is ALVAC or an attenuated canarypox virus wherein said canarypox virus is attenuated through more than 200 serial passages on chick embryo fibroblasts, a master seed therefrom was subjected to four successive plaque purifications under agar, from which a plaque clone was amplified through five additional passages.