Loading patent…
AssigneeMillipore Investment Holdings Limited
InventorsWilliam Kopaciewicz, Ingeborg Cann, Donald G. Sheer
Estimating the term…
Adsorptive sample preparation device and method effective for concentrating, desalting and/or purifying biomolecules. An adsorptive membrane is used to bind biomolecules, which biomolecules can then be eluted with a suitable desorption agent. In a preferred form, the adsorptive filter means is a particle-laden semipermeable membrane having at least one of its major surfaces, preferably both of its major surfaces, covered with a thin fabric. In the method of the invention for concentrating biomolecules or desalting or purify from a solution, the sample reservoir of the above-described adsorptive sample preparation device is filled with an analyte solution, placed in a fixed angle or swinging bucket centrifuge rotor, the solution is driven through the membrane, and analyte obtained as a result of adsorption on the membrane is recovered by washing and then eluting with a suitable desorption agent. A method of sealing the membrane in the device is also set forth.
A centrifugal adsorptive sample preparation device, comprising:
A centrifugal adsorptive sample preparation device, comprising:
A method of sealing an adsorptive membrane in a sample preparation device having a first means for receiving a sample and second means for dispensing said sample, and wherein one of said first and second means comprises a membrane support, comprising:
A polymer bound, particle laden adsorptive membrane effective for selectively adsorbing analyte, said membrane comprising a first surface adapted to be exposed to said analyte, said first surface having a layer of polyester- or polyolefin-based fabric thereon.
An adsorptive centrifugal filtration device comprising:
A method of selectively adsorbing biomolecules having a molecular weight less than about 10,000 from a sample, comprising:
Dependencies are inferred from the claim text.