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OwnerJohn William Redmond, Nicolle Hannah Packer +7inventors
InventorsJohn William Redmond, Nicolle Hannah Packer, Andrew Arthur Gooley, Keith Leslie Williams, Michael Batley, Warren Charles Kerr, Anthony Pisano, Helen Joan Tweeddale, Catherine Anne Cooper
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The present invention consists in methods of preparing sugar derivatives either in isolation or from glycopeptides or glycoproteins. The methods comprise producing sugar hydrazones, sugar pyrazoles, glycosylpyrazones, azoglycan dyes and hydrazoglycan dyes. The present invention also relates to the removal of O-glycans from glycopeptides or glycoproteins, immobilizing reducing sugars onto solid supports and methods to obtain sugars from a glycopeptide or glycoprotein comprising subjecting the glycopeptide or glycoprotein to solid phase Edman degradation followed by separating and characterizing the sugars.
A method for the production of a sugar hydrazone comprising reacting an aqueous hydrazine hydrate with a reducing sugar in the presence of a further base, wherein the reaction does not require addition of acid to form the sugar hydrazone.
A method for the production of an azoglycan dye comprising the following steps:
A method for the removal of O-glycans from a glycopeptide or glycoprotein having O-glycans comprising reacting the glycopeptide or glycoprotein with aqueous hydrazide hydrate in the presence of an additional base at an elevated temperature for a period sufficient to remove the O-glycans in the form of glycan hydrazones without releasing N-glycans from the glycopeptide or glycoprotein.
A method of immobilizing a reducing sugar onto a solid support having primary or secondary amine groups comprising converting the reducing sugar to a sugar hydrazone, converting the amine groups on the solid support to isothiocyanate groups and reacting the sugar hydrazone with the isothiocyanate-substituted support so as to cause the binding of the sugar to the support.
Dependencies are inferred from the claim text.