US 5,831,071 A · Synthesis deprotection analysis and purification of RNA and ribozymes

Abstract

A process for deprotecting RNA 2'-O-alkylsilyl groups comprising, contacting the groups with a solution of anhydrous triethylamine-hydrogen fluoride (aHF-TEA) in triethylamine and N-methylpyrrolidine at between 60.degree. C.-70.degree. C. for 0.25-24 h.

Claims2

Dependent claims

A process for deprotecting RNA 2'-O-alkylsilyl groups comprising, contacting said groups with a solution of anhydrous triethylamine-hydrogen fluoride (aHF-TEA) in triethylamine and N-methylpyrrolidine at between 60.degree. C.-70.degree. C. for 0.25-24 h.

Dependencies are inferred from the claim text.

Facts

Application

08/919,568Filed 29 Aug 1997 · continuation

Priority

14 May 1992

Granted

3 Nov 1998

Term ends

Not estimatedThe source records don’t support an estimate.

Classification45 codes

C12Q 1/6806
Involving nucleic acids: preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C07K 14/005
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from viruses
C12N 15/1003
Processes for the isolation, preparation or purification of DNA or RNA: extracting or separating nucleic acids from biological samples, e.g. pure…
C12N 15/101
By means of a solid support carrier, e.g. particles, polymers: by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Family

Serial884,436Parent of this continuation · filed 14 May 1992

Serial167,586Parent of this continuation-in-part · filed 14 Dec 1993

Serial245,736Parent of this continuation-in-part · filed 18 May 1994

Serial345,516Parent of this continuation-in-part · filed 28 Nov 1994

Serial380,734Parent of this division · filed 30 Jan 1995

Serial434,559Parent of this continuation · filed 2 May 1995

US 5,831,071 AThis patent

From the US filing history. Foreign family members aren’t listed.

Abstract

Facts

Application

08/919,568Filed 29 Aug 1997 · continuation

Priority

14 May 1992

Granted

3 Nov 1998

Term ends

Not estimatedThe source records don’t support an estimate.

Classification45 codes

C12Q 1/6806
Involving nucleic acids: preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C07K 14/005
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from viruses
C12N 15/1003
Processes for the isolation, preparation or purification of DNA or RNA: extracting or separating nucleic acids from biological samples, e.g. pure…
C12N 15/101
By means of a solid support carrier, e.g. particles, polymers: by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Family

Serial884,436Parent of this continuation · filed 14 May 1992

Serial167,586Parent of this continuation-in-part · filed 14 Dec 1993

Serial245,736Parent of this continuation-in-part · filed 18 May 1994

Serial345,516Parent of this continuation-in-part · filed 28 Nov 1994

Serial380,734Parent of this division · filed 30 Jan 1995

Serial434,559Parent of this continuation · filed 2 May 1995

US 5,831,071 AThis patent

From the US filing history. Foreign family members aren’t listed.

Synthesis deprotection analysis and purification of RNA and ribozymes

Life of this patent

Abstract

Claims2

Synthesis deprotection analysis and purification of RNA and ribozymes

Life of this patent

Abstract

Claims2