Method to increase the density of antigen on antigen presenting cells
AssigneeDuke University
InventorsSmita K. Nair, Eli Gilboa
Life of this patent
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Abstract
Disclosed is a method for presenting an antigen in the form of a peptide on the surface of a cell. The method involves inhibiting the activity of an MHC class I pathway-associated component (e.g., a TAP protein or a proteasome or its components) in a cell and contacting the cell with an antigenic peptide to produce a potent antigen presenting cell. The antigen presenting cells of the invention can be administered to a mammal in a method of treating or preventing cancer or infection with a pathogen (e.g., a bacterium or virus). If desired, the antigen presenting cells can be used to stimulate CTL proliferation in vitro, and the resulting effector cells can subsequently be administered to a mammal in a method of therapy.
Claims27
A method of increasing the presentation of a peptide on a mammalian cell, said method comprising inhibiting activity of an MHC class I pathway-associated component in said cell in vitro prior to contacting said cell with said peptide,
- wherein said MHC class I pathway-associated component is a TAP protein or a proteasome.
A mammalian cell in vitro containing an antisense oligonucleotide that reduces expression of an MHC class I pathway-associated protein, wherein said MHC class I pathway-associated protein is a TAP protein.
A cytotoxic T lymphocyte produced by:
- inhibiting activity of an MHC class I pathway-associated component in a mammalian cell in vitro, wherein said MHC class I pathway-associated component is a TAP protein or a proteosome;
- contacting said cell with an antigen, thereby producing an antigen presenting cell;
- and
- contacting a T lymphocyte with said antigen presenting cell in vitro, thereby producing a cytotoxic T lymphocyte.
Dependencies are inferred from the claim text.