Genetic immunization
AssigneesThe Trustees of the University of Pennsylvania, The Wistar Institute
InventorsDavid B. Weiner, William V. Williams, Bin Wang
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Abstract
A method of immunizing an individual against pathogen is disclosed. Also disclosed is a method of treating an individual who has a hyperproliferative disease, or of treating an individual who is infected by a pathogen. Specifically, the individual is injected with bupivacaine along with DNA in an expressible form, the DNA encoding an antigen. The encoded antigen can be from a protein from the pathogen or from a protein associated with the hyperproliferative disease.
Claims17
A method of immunizing an individual against a pathogen comprising the steps of:
- injecting into skeletal muscle tissue of said individual at a site on said individual's body, bupivacaine and a DNA molecule that comprises a DNA sequence that encodes an antigen from said pathogen, said DNA sequence operatively linked to regulatory sequences which control the expression of said DNA sequence;
- wherein said DNA molecule is taken up by cells in said skeletal muscle tissue, said DNA sequence is expressed in said cells and a protective immune response is generated against said antigen.
A method of treating an individual who has a hyperproliferative disease comprising:
- injecting into skeletal muscle tissue of said individual at a site on said individual's body, bupivacaine and a DNA molecule that comprises a DNA sequence that encodes a hyperproliferative disease-associated protein operatively linked to regulatory sequences;
- wherein said DNA molecule is taken up by cells in said skeletal muscle tissue, said DNA sequence is expressed in said cells, and a therapeutically effective immune response is generated against said hyperproliferative disease-associated protein, said immune response being directed at hyperproliferating cells expressing said hyperproliferative disease-associated protein.
A method of treating an individual who is infected by a pathogen comprising:
- injecting into skeletal muscle tissue of said individual at a site on said individual's body, bupivacaine and a DNA molecule that comprises a DNA sequence that encodes an antigen from said pathogen, said DNA sequence operatively linked to regulatory sequences which control the expression of said DNA sequence;
- wherein said DNA molecule is taken up by cells in said skeletal muscle tissue, said DNA sequence is expressed in said cells and a therapeutically effective immune response is generated.
Dependencies are inferred from the claim text.