Loading patent…
AssigneesInstitut Pasteur, Institut Curie, Centre National De La Recherche Scientifique
InventorsFrederic Jaisser, Michel Cohen-Tannoudji, Sylvie Robine, Andre Choulika, Daniel Louvard, Charles Babinet
Estimating the term…
This invention relates to a method of constructing a villin gene hybrid by inserting an I-Sce I restriction site next to or within a gene or cDNA encoding a villin protein. The insertion site of the I-Sce I restriction site is chosen as to provide a first downstream part and a second upstream part from the site, containing at least twelve nucleotides of the gene or cDNA encoding the villin protein. Furthermore, the insertion of the restriction permits a high frequency of homologous recombination events. The villin gene hybrid may be used to transfect eukaryotic cells, and particularly, embryonic stem cells.
A method of making a villin gene hybrid containing a locus for homologous recombination, wherein the method comprises inserting an I-SceI restriction site adjacent to or within a villin gene or cDNA encoding villin protein, such that at least a portion of the locus for homologous recombination is contained within the villin gene or cDNA encoding villin protein, and wherein said locus comprises the I-SceI restriction site and at least twelve nucleotides upstream and downstream of the I-SceI restriction site providing homology for homologous recombination.
A hybrid nucleotide sequence comprising an I-SceI restriction site adjacent to or within a villin gene or cDNA encoding a villin protein, such that at least a portion of a locus for homologous recombination is contained within the villin gene or cDNA encoding villin protein, wherein said locus comprises the I-SceI restriction site and at least twelve nucleotides upstream and downstream of the I-SceI restriction site.
Dependencies are inferred from the claim text.