US 5,830,709 AGranted

Detection method for homologous portions of a class of substances

OwnerRoger E. Benson, James L. Catalfamo +1inventors

InventorsRoger E. Benson, James L. Catalfamo, W. Jean Dodds

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Abstract

The invention provides a method of detecting a class of substances, by providing an assay configuration which allows for reaction with a homologous portion common to all members of the class. The configuration utilizes a capture reactant to immobilize class members, revealing previously unrecognizable reaction sites on the class. The capture reactant interacts with a homologous portion on the class members. The immobilized class is then detected, such as by the use of a sandwich antibody directed to another homologous portion of the class now exposed for reaction, or directed to specific portions of specific members of the class that have been exposed for reaction. These reaction sites were unrecognizable prior to immobilization of the class members with the capture reactant.

Claims14

Dependent claims

An immunoassay for detecting members of a class of molecules in a sample, each member of the class having a homologous portion common to members of the class, each member of the class being from a different species, said method comprising:

a. obtaining a panel of antibodies generated to recognize and bind a plurality of members of the class, and which were not generated to recognize and bind the member included in the sample;
b. selecting an antibody from (a) having a high binding affinity for the member at the class included in the sample
c. attaching the antibody selected in (b) to an immunological reaction surface;
d. adding the sample to the immunological reaction surface under conditions so that the attached antibody recognizes and binds a member of the class so as to form a complex, the attached antibody reacting with and binding the homologous portion of the class;
and
e. detecting the complex so bound thereby detecting members of a class.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application: 08/035,452Filed 23 Mar 1993 · continuation in part
Priority: 27 Oct 1989
Granted: 3 Nov 1998
Term ends: Not estimatedThe source records don’t support an estimate.

Classification5 codes

C07K 16/36
Against material from animals or humans: against blood coagulation factors
G01N 33/86
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing:…
G01N 2333/755
Assays involving non-enzymic blood coagulation factors: factors VIII, e.g. factor VIII C [AHF], factor VIII Ag [VWF]
C07K
Peptides

Family

Serial428,161Parent of this continuation-in-part · filed 27 Oct 1989
Serial604,885Parent of this continuation-in-part · filed 26 Oct 1990
US 5,196,311 AParent of this continuation-in-part · filed 23 Mar 1993
US 5,202,264 AParent of this continuation-in-part
US 5,830,709 AThis patent

From the US filing history. Foreign family members aren’t listed.