Live, recombinant listeria monocytogenes and production of cytotoxic T-cell response
AssigneeThe Trustees of the University of Pennsylvania
InventorsDaniel A. Portnoy, Yvonne Paterson
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Abstract
Vaccines comprising an attenuated strain of Listeria spp. expressing a selected foreign antigen and methods of eliciting protective immunity by administering an effective amount of the vaccine are provided. The vaccines of the present invention are designed specifically to elicit strong cell-mediated immunity, and are particularly useful for protection in infections which can apparently persist and spread in the presence of neutralizing antibodies invoked by humoral immunity.
Claims15
A vaccine comprising recombinant Listeria monocytogenes that expresses and secretes a fusion protein, the vaccine causing the production of a cytotoxic T-cell response in a vertebrate, wherein said fusion protein comprises a foreign antigen of an infectious agent and Listeriolysin O protein or a foreign antigen of an infectious agent and a signal sequence from a listerial protein.
A recombinant Listeria monocytogenes culture capable of expressing and secreting a fusion protein comprising viable Listeria monocytogenes transformed with an expression vehicle containing DNA encoding at least a portion of Listeriolysin O protein in translational reading frame with DNA encoding a foreign antigen of an infectious agent.
Dependencies are inferred from the claim text.