Active immunization using a siderophore receptor protein
AssigneeWillmar Poultry Company, Inc.
InventorsDaryll A. Emery, Darren E. Straub, Richard Huisinga, Beth A. Carlson
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Abstract
The invention provides a vaccine for immunizing poultry and other animals against infection by a gram-negative bacteria, and a method of immunizing an animal using the vaccine. The vaccine may contain purified siderophore receptor proteins derived from a single strain or species of gram-negative bacteria or other organism, which are cross-reactive with siderophores produced by two or more strains, species or genera of gram-negative bacteria. The invention further provides a process for isolating and purifying the siderophore receptor proteins, and for preparing a vaccine containing the proteins. Also provided is a method for diagnosing gram-negative sepsis.
Claims16
A vaccine for use in immunizing an animal against an infection by a gram-negative bacteria, said vaccine comprising:
- (a) at least four siderophore receptor proteins extracted from an outer membrane of at least two different serotypes of bacteria of the Pasteurellaceae family;
- (b) a non-iron-regulated outer membrane protein extracted from a bacteria selected from the group of bacteria in the families of Enterobacteriaceae and Pasteurellaceae and, having a molecular weight of about 34-38 kDa;
- and
- (c) a physiologically acceptable carrier.
A method for immunizing an animal against an infection by a gram-negative bacteria, comprising:
- (a) administering to the animal a vaccine comprising:
- (i) at least four siderophore receptor proteins extracted from an outer membrane of at least two different serotypes of bacteria of the Pasteurellaceae family;
- (ii) a non-iron-regulated outer membrane protein extracted from a bacteria selected from the group of bacteria in the families of Enterobacteriaceae and Pasteurellaceae and, having a molecular weight of about 34-38 kDa;
- and
- (iii) a physiological acceptable carrier.
Dependencies are inferred from the claim text.