Recombinant mycobacterial vaccines
AssigneeWhitehead Institute for Biomedical Research
InventorsAnna Aldovini, Richard A. Young
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Abstract
The present invention relates to recombinant mycobacteria, particularly recombinant M. bovis BCG, which express heterologous DNA encoding a product (protein or polypeptide) of interest, such a protein or polypeptide (e.g., an antigen) against which an immune response is desired, or a cytokine.
Claims5
A method of inducing an immune response in a mammalian host against one or more pathogens, comprising administering to the host a live, recombinant mycobacterium, the recombinant mycobacterium having incorporated therein a plasmid comprising:
- DNA of interest encoding at least one protein antigen for each of said pathogens fused to a mycobacterial heat shock gene promoter or a mycobacterial stress protein gene promoter and translational start site in such a manner that expression of the DNA of interest is under the control of the promoter,
- wherein the protein antigen is produced by the recombinant mycobacterium replicating in the host.
A vaccine comprising a live, recombinant mycobacterium having DNA of interest which is expressed extrachromosomally under the control of a mycobacterial heat shock gene promoter or a mycobacterial stress protein gene promoter and an appropriate carrier.
Dependencies are inferred from the claim text.