Adipogenic differentiation of human mesenchymal stem cells
AssigneeOsiris Therapeutics, Inc.
InventorMark F. Pittenger
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Abstract
A composition which comprises human mesenchymal stem cells which have the potential to differentiate into cells of more than one connective tissue type and a composition which induces cells from the mesenchymal stem cell population to differentiate into the adipogenic lineage, and a process for inducing such differentiation. The composition for inducing such differentiation comprises a glucocorticoid and a compound which stimulates cAMP production or inhibits cAMP degradation (such as a phosphodiesterase inhibitor). The process can further include isolating the adipocytes from remaining hMSCs.
Claims14
A composition which comprises mesenchymal stem cells which have been isolated from a human and which have potential to differentiate into cells of more than one connective tissue type and a substance in an amount effective to induce said stem cells to differentiate into the adipogenic lineage.
A process for inducing human mesenchymal stem cells to differentiate into adipocytes, comprising:
- contacting human mesenchymal stem cells with (i) a glucocorticoid and (ii) a compound which elevates intracellular levels of cAMP, said glucocorticoid and said compound which elevates intracellular levels of cAMP being employed in an amount sufficient to induce human mesenchymal stem cells to differentiate into adipocytes.
A process for inducing human mesenchymal stem cells to differentiate into adipocytes, comprising:
- contacting human mesenchymal stem cells with (i) a glucocorticoid and (ii) a compound which inhibits degradation of cAMP, said glucocorticoid and said compound which inhibits degradation of cAMP being employed in an amount sufficient to induce human mesenchymal stem cells to differentiate into adipocytes.
Dependencies are inferred from the claim text.