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US 5,827,690 AGranted
Transgenic production of antibodies in milk
AssigneeGenzyme Transgenics Corporation
InventorsHarry Meade, Paul DiTullio, Daniel Pollock
Life of this patent
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Abstract
A method for the production of monoclonal antibodies in mammal's milk, through the creation of transgenic animals that selectively express foreign antibody genes in mammary epithelial cells.
Claims13
A high level expression method for providing a heterologous and assembled immunoglobulin, in the milk of a transgenic mammal comprising:
- obtaining milk from a transgenic mammal having introduced into its germline a heterologous immunoglobulin protein-coding sequence operatively linked to a promoter sequence that results in the preferential expression of said protein-coding sequence in mammary gland epithelial cells, thereby providing said heterologous and assembled immunoglobulin in the milk of said mammal, wherein said heterologous and assembled immunoglobulin is a functional configuration and is produced at level of at least about 1 mg/ml in the milk of said mammal.
A high level expression method for providing a heterologous and assembled immunoglobulin, in the milk of a transgenic goat comprising:
- obtaining milk from a transgenic goat having introduced into its germline a heterologous immunoglobulin protein-coding sequence operatively linked to a promoter sequence that results in the preferential expression of said protein-coding sequence in mammary gland epithelial cells, thereby providing said heterologous and assembled immunoglobulin in the milk of said goat, wherein said heterologous and assembled immunoglobulin is a functional configuration and is produced at levels of at least about 1 mg/ml in the milk of said goat.
Dependencies are inferred from the claim text.