Homocysteine assay
AssigneeAxis Biochemicals AS
InventorErling Sundrehagen
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Abstract
The invention relates to a method for assaying homocysteine in a sample such as blood, plasma, or urine, which comprises the steps of contacting the sample with a homocysteine converting enzyme and at least one substrate for the enzyme other than homocysteine, and without chromatographic separation, assessing a non-labelled analyte selected from a homocysteine co-substrate and the homocysteine conversion products of the enzymic conversion of homocysteine by said enzyme.
Claims23
A method for assaying homocysteine in a sample, said method comprising the steps of contacting said sample with a homocysteine converting enzyme other than S-adenosyl homocysteine hydrolase and at least one substrate for said enzyme other than homocysteine, and without chromatographic separation assessing a non-labelled analyte selected from a homocysteine co-substrate and the homocysteine conversion products of the enzymic conversion of homocysteine by said enzyme using immunological techniques.
A method for assaying for a non-antigenic target substance in a sample, said method comprising enzymatically converting said target substance to an antigenic analyte and assessing said analyte by an immunoassay procedure.
An analytical kit for use in a method for assaying homocysteine in a sample, said method comprising the steps of contacting said sample with a homocysteine converting enzyme and at least one substrate for said enzyme other than homocysteine, and without chromatographic separation assessing a non-labelled analyte selected from a homocysteine substrate and the homocysteine conversion products of the enzymic conversion of homocysteine by said enzyme using immunological techniques, said kit comprising:
- a homocysteine converting enzyme;
- a substrate, other than homocysteine, for the homocysteine conversion reaction catalysed by said enzyme;
- a signal forming agent;
- and, optionally, means for signal assessment.
Dependencies are inferred from the claim text.