In vitro cornea equivalent model

The method of producing a cornea equivalent comprising:

• (a) culturing endothelial cells to form an inner endothelial cell layer;
• (b) mixing stromal cells with collagen to achieve a stromal cell-collagen mixture, wherein said stromal cells are derived from fibroblast cells;
• (c) contacting said inner endothelial cell layer of step (a) with said stromal cell-collagen mixture of step (b), thereby forming a middle stromal cell-collagen mixture layer provided on said inner layer;
• (d) culturing said inner endothelial cell layer and said middle layer;
• (e) contacting corneal epithelial cells onto said middle layer of step (d);
• (f) culturing said corneal epithelial cells with said middle layer until said middle layer is covered with an external layer of epithelial cells;
• and,
• (g) continue culturing said inner, middle, and external layers to form a cornea equivalent.