Plant promoter
AssigneeWashington University
InventorsRoger N. Beachy, Maitrayee Bhattacharyya
Life of this patent
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Abstract
A genome length transcript promoter from a badnavirus, rice tungro bacilliform virus (RTBV), is disclosed and its DNA sequence provided. This promoter drives expression specifically in vascular tissues of plants. This promoter sequence may be utilized in a chimeric gene to drive the tissue specific expression of a foreign structural gene in vascular tissue of transgenic plants.
Claims24
An isolated genome-length transcript promoter from rice tungro bacilliform virus.
A chimeric gene that functions in plant cells comprising:
- a genome-length transcript promoter from rice tungro bacilliform virus;
- a structural DNA sequence that is heterologous with respect to said promoter;
- and
- a 3' non-translated DNA sequence which functions in plants to cause the addition of polyadenylated nucleotides to the 3' end of the transcribed sequence.
An isolated genome-length transcript promoter from rice tungro bacilliform virus capable of driving transcription of a structural DNA sequence operably coupled thereto in the vascular bundles of a plant.
A transformed plant cell comprising a chimeric gene comprised of:
- a genome-length transcript promoter from rice tungro bacilliform virus;
- a structural DNA sequence that is heterologous with respect to said promoter;
- and
- a 3' non-translated DNA sequence which functions in plants to cause the addition of polyadenylated nucleotides to the 3' end of the transcribed sequence.
A transgenic plant comprising a gene regulated by a genome-length transcript promoter from rice tungro bacilliform virus, wherein said gene is heterologous with respect to said promoter.
A method of making a transgenic plant comprising:
- introducing a chimeric gene into plant cells of a plant, said chimeric gene comprised of a genome-length transcript promoter from rice tungro bacilliform virus, a structural DNA sequence that is heterologous with respect to said promoter, and a 3' non-translated DNA sequence which functions in plants to cause the addition of polyadenylated nucleotide to the 3' end of the transcribed sequence;
- culturing said plant cells in a culturing medium containing a selection agent to identify plant cells containing said chimeric gene;
- and
- regenerating said plant cells containing said chimeric gene into whole plants.
A method for expressing a foreign structural gene in a plant under the regulatory control of a genome-length transcript promoter from rice tungro bacilliform virus comprising:
- transforming plant cells of said plant with a transformation vector comprised of a foreign structural gene under the regulatory control of a genome-length transcript promoter from rice tungro bacilliform virus;
- and
- growing said plant cells under conditions whereby said foreign structural gene is expressed under the regulatory control of said genome-length transcript promoter from rice tungro bacilliform virus.
A method for causing the expression of a structural DNA coding sequence in the vascular bundles of a plant, the method comprising;
- placing said structural DNA coding sequence under the regulatory control of a genome-length transcript promotor from rice tungro bacilliform virus, said structural DNA coding sequence being heterologous with respect to said promoter.
A DNA sequence consisting essentially of an isolated genome-length transcript promoter from rice tungro bacilliform virus.
Dependencies are inferred from the claim text.