US 5,824,798 A · Genetically engineered modification of potato to obtain amylopectin-type starch

Abstract

Genetically engineered modification of potato for suppressing formation of amylose-type starch is described. Three fragments for insertion in the antisense direction into the potato genome are also described. Moreover, antisense constructs, genes and vectors comprising said antisense fragments are described. Further a promoter for the gene coding for formation of granule-bound starch synthase and also the gene itself are described. Also cells, plants, tubers, microtubers and seeds of potato comprising said antisense construct are described. Finally, amylopectin-type starch, both native and derivatized, derived from the potato that is modified in a genetically engineered manner, as well as a method of suppressing amylose formation in potato are described.

Claims8

Dependent claims

A process for producing an amylopectin-type starch comprising:

obtaining a potato tissue which has been transformed by introducing into the genome of the potato tissue a gene construct comprising a promoter and a fragment of the potato gene which codes for the formation of granule-bound starch synthase inserted in the anti-sense direction, wherein said fragment essentially has a nucleotide sequence which is selected from the group consisting of SEQ ID No. 1, SEQ ID No.2 and SEQ ID No. 3;
growing the transformed potato tissue to produce a potato plant containing potato tubers;
producing at least one potato from said potato tubers;
and
separating starch from said potato, wherein said starch is an amylopectin-type starch which is essentially free of amylose.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

08/470,720Filed 6 Jun 1995 · continuation

Priority

SE 900409621 Dec 1990

Granted

20 Oct 1998

Term ends

Not estimatedThe source records don’t support an estimate.

Classification8 codes

C12N 15/1137
Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming…: against enzymes
C12N 9/1051
Glycosyltransferases: hexosyltransferases
C12N 15/8245
Involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine: involving modified carbohydrate or sugar…
C12P 19/04
Preparation of compounds containing saccharide radicals: polysaccharides, i.e. compounds containing more than five saccharide radicals attached to…

Genetically engineered modification of potato to obtain amylopectin-type starch

Life of this patent

Genetically engineered modification of potato to obtain amylopectin-type starch

Life of this patent

Abstract

Claims8