US 5,824,790 AGranted

Modification of starch synthesis in plants

AssigneeZeneca Limited

InventorsPeter Lewis Keeling, Mary E. Knight, Hanping Guan

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Abstract

A method of producing a plant with novel starch-synthesising ability comprises stably incorporating into the genome of a recipient plant at least one target gene encoding an enzyme involved in a starch or glycogen biosynthetic pathway. A plant with novel starch-synthesising ability may have novel starch quality (eg altered fine structure). Starch or glycogen biosynthetic enzymes include soluble starch synthase, branching enzyme, glycogen synthase, ADP-glucose pyrophosphorylase, self-glucosylating protein, glycogenin and amylogenin. Mutants involving said enzymes for use in this method are described, including specific gene-dosage combinations which provide novel starch fine structures and starch properties. DNA constructs for use in this method are described, as well as plants transformed with said DNA constructs, the seeds and progeny of such plants, and hybrids whose pedigree includes such plants.

Claims6

Dependent claims

An isolated nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide having soluble starch synthase activity wherein said polypeptide is encoded by a maize gene, said nucleotide sequence being selected from the group consisting of:

(a) a sequence comprising the nucleotide sequence in SEQ ID NO:
1;
(b) a nucleotide sequence encoding the polypeptide having soluble starch synthase activity which is encoded by the nucleotide sequence in SEQ ID NO:
1;
(c) a nucleotide sequence comprising the nucleotide sequence of the maize cDNA clone contained in the deposit in the National Collection of Industrial and Marine Bacteria Limited with Accession Number 40651;
(d) a nucleotide sequence encoding the polypeptide having soluble starch synthase activity which is encoded by the maize cDNA clone contained in the deposit in the National Collection of Industrial and Marine Bacteria Limited with Accession Number 40651;
(e) a nucleotide sequence encoding the polypeptide having soluble starch synthase activity wherein said polypeptide comprises the amino acid sequence consisting of SEQ ID NO:
33, and,
(f) a nucleotide sequence encoding the polypeptide having soluble starch synthase activity wherein said polypeptide comprises the amino acid sequence consisting of SEQ ID NO:
22 and SEQ ID NO:
26.

8 more elements

Dependencies are inferred from the claim text.

Facts

Application: 08/572,951Filed 15 Dec 1995 · continuation in part
Priority: 21 Jun 1994
Granted: 20 Oct 1998
Term ends: Not estimatedThe source records don’t support an estimate.

Classification13 codes

C12N 15/8243
With non-agronomic quality traits, e.g. for industrial processing; Value added, non-agronomic traits: involving biosynthetic or metabolic…
A21D 2/186
Carbohydrates: starches; Derivatives thereof
A23G 1/423
Containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins: containing microorganisms or enzymes
A23G 1/56
Cocoa products, e.g. chocolate; Substitutes therefor: liquid products; Solid products in the form of powders, flakes or granules for making liquid…