Process for the production of expression vectors comprising at least one stochastic sequence of polynucleotides

A process for the production of an expression vector capable of producing a transcription product or a translation product comprising at least one stochastic sequence of polynucleotides, comprising the steps of:

• linearizing an expression vector;
• reacting said linearized expression vector with terminal transferase enzyme in the presence of desired ratios of deoxynucleotide-triphosphates of guanine, cytosine, thymidine, and adenine to form a stochastic polynucleotide sequence at each 3' extremity of said linearized vector;
• hybridizing said stochastic polynucleotide sequence at a 3' extremity of said linearized vector;
• and
• synthesizing a second strand from said 3' ends of said hybridized vector by incubating with polymerase.

A process for the production of a library of expression vectors capable of producing a transcription product or a translation product, said vectors comprising at least one stochastic sequence of polynucleotides, comprising the steps of:

• producing at least one stochastic sequence of polynucleotides;
• ligating said stochastic sequence of polynucleotides into an expression vector;
• transforming a competent clone with said ligated expression vector;
• culturing said transformed clone;
• screening and/or selecting said transformed clone in order to isolate a clone expressing a stochastic polynucleotide leading to the synthesis of a transcription product or a translation product;
• isolating said selected or screened transformed clone;
• and
• isolating the expression vector cultured in said selected or screened transformed clone so identified.