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AssigneeMerck Patent Gesellschaft mit beschrankkter Haftung
InventorsSiegfried Scherer, Martin Loessner
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The invention relates to a detection procedure for bacteria of the genus Listeria, comprising the steps: (a) provision of a DNA vector prepared by means of recombination techniques, comprising a genetic system comprising DNA which encodes the expression of one or more proteins, the proteins not being a gene product of bacteria of the genus Listeria and it being possible to determine the presence of the proteins by a detection reaction, and the DNA vector infecting the bacteria of the genus Listeria and it being possible in this way to transfer the genetic system to the bacteria; (b) mixing of the sample with said DNA vector under conditions which allow an infection of bacteria of the genus Listeria by the DNA vector; (c) expression of the detectable proteins in the bacteria of the genus Listeria; (d) detection of the detectable proteins, the presence of bacteria of the genus Listeria being detected, and to recombinant DNA vectors and reagent compositions suitable for this detection procedure.
An infectious recombinant bateriophage derived from a myophage of morphotype A1, species A511, comprising a marker gene exogenous to bacteria of the genus Listeria, wherein said gene encodes a marker gene product.
Dependencies are inferred from the claim text.