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AssigneeGenentech, Inc.
InventorsLisa J. Garrard, Dennis J. Henner, Steven Bass, Ronald Greene, Henry B. Lowman, James A. Wells, David J. Matthews
Estimating the term…
A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.
A method for selecting novel binding polypeptides comprising:
A group of phagemid particles, each particle comprising a replicable expression vector comprising a transcription regulatory element operably linked to a gene fusion encoding a fusion protein wherein the gene fusion comprises a first gene encoding a polypeptide, and a second gene encoding at least a portion of a phage coat protein, wherein a DNA tripler codon encoding an mRNA suppressible terminator codon selected from UAG, UAA and UGA is inserted between the fused ends of the first and second genes, or is substituted for an amino acid encoding triplet codon adjacent to the gene fusion junction, wherein no more than a minor amount of phagemid particles in said group display one or more copies of the fusion protein on the surface of the particle.
A method for selecting novel binding polypeptides comprising:
A method for selecting novel binding polypeptides comprising:
A method for selecting novel binding polypeptides comprising:
A group of phagemid particles, each particle comprising a replicable expression vector comprising a transcription regulatory element operably linked to a gene fusion encoding a fusion protein wherein the gene fusion comprises a first gene encoding a polypeptide, and a second gene encoding at least a portion of a phage coat protein, wherein no more than a minor amount of phagemid particles in said group display one or more copies of the fusion protein on the surface of the particle.
A method for selecting novel binding polypeptides comprising steps of:
Dependencies are inferred from the claim text.