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AssigneeMount Sinai School of Medicine of the City University of New York
InventorsPeter Palese, Adolfo Garcia-Sastre
Estimating the term…
Recombinant negative-strand viral RNA templates are described which may be sed with purified RNA-directed RNA polymerase complex to express heterologous gene products in appropriate host cells and/or to rescue the heterologous gene in virus particles. The RNA templates are prepared by transcription of appropriate DNA sequences with a DNA-directed RNA polymerase. The resulting RNA templates are of the negative-polarity and contain appropriate terminal sequences which enable the viral RNA-synthesizing apparatus to recognize the template. Bicistronic mRNAs can be constructed to permit internal initiation of translation of viral sequences and allow for the expression of foreign protein coding sequences from the regular terminal initiation site, or vice versa.
A vaccine comprising a chimeric negative strand RNA virus the genome of which contains the reverse complement of an MRNA coding sequence operatively linked to a polymerase binding site of the negative strand RNA virus, and a pharmaceutically acceptable carrier.
A composition comprising a chimeric negative strand RNA virus the genome of which contains the reverse complement of an mRNA coding sequence which encodes a human immunodeficiency virus epitope operatively linked to a polymerase binding site of the negative strand RNA virus, and a carrier.
Dependencies are inferred from the claim text.