VSV G pseusdotyped retroviral vectors
AssigneesThe Regents of the University of California, Chiron Viagene, Inc.
InventorsJiing-Kuan Yee, Nobuhiko Emi, Theodore Friedmann, Douglas J. Jolly, Jack R. Barber
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Abstract
An enveloped vector particle contains gag and pol proteins from a retrovirus, a nucleic acid sequence and an envelope that includes VSV G envelope glycoprotein. The vector particle can be used to introduce nucleic acids into cells.
Claims22
An enveloped vector particle comprising:
- gag and pol proteins from a retrovirus;
- a nucleic acid sequence associated with said vector particle;
- an envelope having vesicular stomatitis virus (VSV) G envelope glycoprotein therein.
A cell line that stably expresses or carries gag/pol, transfected or transduced with a nucleic acid molecule encoding a retroviral vector and a nucleic acid molecule encoding a VSV G protein, the cell line being capable of producing functional vector particles.
A 293 cell stably expressing gag/pol.
A method of making a VSV G pseudotyped virus, comprising:
- introducing into a cell line that stably expresses gag/pol a VSV G protein expression vector and a retroviral vector construct, the resulting cell line being capable of producing vector particles comprising VSV G protein.
A method of making a VSV G pseudotyped virus, comprising:
- (a) cotransfecting or sequentially transfecting a cell with a vector that directs the expression of gag/pol and a retroviral vector construct;
- and
- (b) introducing a VSV G protein expression vector into the cotransfected or sequentially transfected cell, the resulting cell being capable of producing vector particles comprising VSV G protein.
Dependencies are inferred from the claim text.