Process for the production of stochastically-generated peptides,polypeptides or proteins having a predetermined property

A process for the production of a peptide, polypeptide, or protein having a predetermined property, comprising the steps of:

• producing a population of at least partially stochastic synthetic polynucleotide sequences;
• introducing the population of at least partially stochastic polynucleotide sequences into host cells to produce transformed host cells;
• cultivating the transformed host cells to produce peptides, polypeptides, or proteins expressed by at least some of the stochastic polynucleotide sequences;
• carrying out screening and/or selection methods on said transformed host cells to identify clones producing the peptide, polypeptide, or protein having the predetermined property;
• isolating the clones so identified;
• and
• growing the isolated clones in a manner so as to produce the peptide, polypeptide, or protein having the predetermined property.

A process for the detection or titration of a ligand using a peptide, polypeptide, or protein having a predetermined property, comprising the steps of:

• producing by synthetic polynucleotide coupling, a population of stochastically generated polynucleotide sequences;
• forming a library of expression vectors containing said population of stochastically generated polynucleotide sequences;
• introducing the vectors into host cells;
• culturing the host cells containing the vectors to produce peptides, polypeptides, or proteins encoded by the stochastically generated polynucleotide sequences;
• carrying out screening or selection on said host cells, to identify a peptide, polypeptide, or protein produced by the host cells having the predetermined property;
• contacting the peptide, polypeptide, or protein with two or more concentrations of a ligand;
• and
• determining the amount of peptide, polypeptide or protein bound at each concentration of ligand.

A process for the detection or titration of a ligand using a peptide, polypeptide, or protein having a predetermined property, comprising the steps of:

• producing a population of at least partially stochastic synthetic polynucleotide sequences;
• introducing the population of at least partially stochastic polynucleotide sequences into host cells to produce transformed host cells;
• cultivating the transformed host cells to produce peptides, polypeptides, or proteins expressed by at least some of the stochastic polynucleotide sequences;
• carrying out screening and/or selection methods on said transformed host cells to identify clones producing the peptide, polypeptide, or protein having the predetermined property;
• contacting the peptide, polypeptide, or protein with two or more concentrations of a ligand;
• and
• determining the amount of peptide, polypeptide or protein bound at each concentration of ligand.

A method of producing a peptide, polypeptide or protein having a predetermined property, comprising:

• (a) producing a population of peptides, polypeptides or proteins encoded by stochastic polynucleotide sequences;
• (b) screening said population of peptides, polypeptides or proteins for said predetermined property under conditions which allow detection of one or more peptides, polypeptides or proteins having said predetermined property;
• (c) isolating the polynucleotide sequence encoding said one or more peptides, polypeptides or proteins having said predetermined property;
• and
• (d) producing said peptide polypeptide or protein.