US 5,817,025 AGranted

Method for diagnosing malignancy diseases

OwnerSergei Grigorevich Alekseev, Nikolai Borisovich Brandt +3inventors

InventorsSergei Grigorevich Alekseev, Nikolai Borisovich Brandt, Galina Aleksandrovna Mironova, Hiroshi Akimoto, Keiko Akimoto

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Abstract

The method for diagnosing malignancy diseases involving analysis of blood plasma by laser correlational spectroscopy (LSC) techniques includes the steps of: preparing a weak diluted solution of native blood plasma, determining a spectral density of light scattering intensity fluctuations within the frequency range between 1 and 1000 Hz, distinguishing a spectrum core and making a diagnosis from a frequency of a maximum and an intensity-to-half-width ratio of a spectrum core envelope, a malignancy disease being detected when the diagnostic parameters are less than the appropriate rates. The apparatus for diagnosing malignancy diseases includes a nephelometer and a series-connected correlation spectrum analyzer. The nephelometer further includes a second scattered light detector disposed symmetrically about a first scattered light detector, a signal multiplier whose inputs are coupled to outputs of the first and second scattered light detectors, a low pass filter whose input is coupled to an output of the signal multiplier, and an output is coupled to a correlator of the spectrum analyzer, a conventional ampoule for a solvent being used as a measuring cuvette in the nephelometer.

Claims1

Dependent claims

A method for diagnosing malignancy diseases comprising the steps of:

(a) preparing a blood plasma solution and placing said blood plasma solution in a measuring cuvette;
(b) generating a primary laser beam and directing said primary laser beam to said measuring cuvette;
(c) recording spectra of light scattering intensity fluctuations in the frequency shift range between 1 Hz and 1000 Hz as output signals of a pair of light detecting means disposed symmetrically about the measuring cuvette and at an angle 90.degree. with respect to the direction of the primary laser beam;
(d) processing said signals in a two-channel correlational detector to define an autocorrelation function of the light scattering intensity fluctuations;
(e) determining a spectral density of a light scattering intensity by processing said autocorrelation function of the light scattering intensity fluctuations in a Fourier transformer;
(f) calculating a spectrum core of said spectral density of the light scattering intensity and determining an envelope of the spectrum core;
(g) defining values of a frequency of a maximum, an intensity and a half-width of said spectrum core envelope;
(h) defining a value of intensity-to-half-width ratio;
(i) comparing the values of said frequency of the envelope maximum and said intensity-to-half-width ratio to respective values for a verified reference group of practically healthy individuals;
and
(j) diagnostically detecting malignancy when said values of the frequency of the envelope maximum and the intensity-to-half-width ratio are less than the respective values for a verified reference group of practically healthy individuals.

8 more elements

Dependencies are inferred from the claim text.