Eukaryotic layered vector initiation systems
OwnerThomas W. Dubensky, Jr., John M. Polo +2inventors
InventorsThomas W. Dubensky, Jr., John M. Polo, Douglas J. Jolly, David A. Driver
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Abstract
The present invention provides compositions and methods for utilizing recombinant alphavirus vectors. Also disclosed are compositions and methods for making and utilizing eukaryotic layered vector initiation systems.
Claims25
A method of stimulating in an animal an immune response to an antigen comprising introducing into susceptible target cells a eukaryotic layered vector initiation system comprising a eukaryotic promoter 5' of viral cDNA which initiates within said cell the 5' to 3' synthesis of RNA from said cDNA, wherein said RNA comprises a vector construct which autonomously amplifies in said cell and expresses a heterologous nucleic acid sequence, wherein said heterologous nucleic acid sequence encodes an antigen or modified form thereof which stimulates an immune response within an animal.
A eukaryotic layered vector initiation system, comprising a eukaryotic promoter 5' of viral cDNA which initiates within a susceptible target cell the 5' to 3' synthesis of RNA from said cDNA, wherein said RNA comprises a vector construct which autonomously amplifies ill a cell and expresses a heterologous nucleic acid sequence which encodes an antigen or modified form thereof that stimulates an immune response within an animal.
Dependencies are inferred from the claim text.