Process for the production of stochastically-generated transcription or translation products

A process for the production of a transcription product or a translation product, comprising the steps of:

• producing a diverse population of stochastic polynucleotide sequences;
• inserting said stochastic polynucleotide sequences into expression vectors to form a diverse population of expression vectors;
• transforming or transfecting competent clones with said diverse population of expression vectors comprising said stochastic polynucleotide sequences;
• amplifying said transformed or transfected competent clone;
• screening and/or selecting said transformed or transfected clones in order to isolate a clone expressing a stochastic polynucleotide capable of synthesizing a transcription product or a translation product having the predetermined property;
• isolating said selected or screened transformed clone;
• isolating said stochastic polynucleotide sequence which encodes the identified transcription product or translation product;
• using the isolated stochastic polynucleotide sequence so as to produce the transcription product or translation product having the predetermined property.

A process for the production of a polynucleotide comprising,

• producing in an appropriate buffer a diverse population of stochastic polynucleotide sequences;
• inserting said stochastic polynucleotide sequences into vectors to form a diverse population of vectors;
• introducing said diverse population of vectors into host cells in a manner to produce a diverse population of transformed host cells;
• producing independent clones of the host cells so produced;
• screening and/or selecting said independent clones of the host cells to identify host cells comprising a stochastic polynucleotide sequence having at least one desired property;
• and
• isolating said stochastic polynucleotide sequence from the selected or screened clones of host cells.

A process for the production of an RNA comprising,

• producing in an appropriate buffer a diverse population of stochastic polynucleotide sequences;
• inserting said stochastic polynucleotide sequences into vectors to form a diverse population of vectors;
• introducing said diverse population of vectors into host cells in a manner to produce a diverse population of transformed host cells;
• producing independent clones of transformed or transfected host cells;
• screening and/or selecting said independent clones of the host cells to identify host cells comprising a stochastic polynucleotide sequence capable of producing RNA having at least one desired property;
• and
• isolating said stochastic polynucleotide sequence from the selected or screened clones of host cells.

A method of identifying a polynucleotide having a binding property to a ligand, comprising:

• (a) synthesizing a population of stochastic polynucleotide sequences;
• (b) inserting said population of stochastic polynucleotide sequences into a population of vectors to form a population of vectors containing stochastic polynucleotide sequences;
• (c) expressing in host cells said population of vectors containing stochastic polynucleotide sequences to produce a diverse population of expressed polynucleotides, and
• (d) screening said diverse population of polynucleotides with a ligand under conditions which allow binding and detection one or more polynucleotides having said binding property to said ligand.

A method of isolating a polynucleotide having a binding property to a ligand, comprising:

• (a) synthesizing a population of stochastic polynucleotide sequences;
• (b) inserting said population of stochastic polynucleotide sequences into a population of vectors to form a population of vectors containing stochastic polynucleotide sequences;
• (c) expressing in host cells said population of vectors containing stochastic polynucleotide sequences to produce a diverse population of expressed polynucleotides, and
• (d) screening said diverse population of polynucleotides with a ligand under conditions which allow binding and detection of one or more polynucleotides to said ligand, and
• (e) isolating the stochastic polynucleotide sequence or sequences having said binding property to said ligand.