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AssigneeMark A. Emalfarb
InventorsMark Aaron Emalfarb, Arie Ben-Bassat, Richard P. Burlingame, Vladimir Mikhaylovich Chernoglazov, Oleg Nicolaevich Okounev, Philip T. Olson, Arkady Panteleimonovich Sinitsyn, Irina Vladimirovna Solovjeva
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The subject invention relates to novel compositions of neutral and/or alkaline cellulase and methods for obtaining neutral and/or alkaline cellulase compositions from Chrysosporium cultures, in particular Chrysosporium lucknowense. This invention also provides mutants and methods of generating mutants of Chrysosporium capable of producing neutral and/or alkaline cellulose. This invention also relates to the genes encoding the enzymes comprising the neutral and/or alkaline cellulase composition. In addition, this invention provides methods of culturing Chrysosporium to produce neutral and/or alkaline cellulases. The neutral and/or alkaline cellulase compositions of the subject invention can be used in a variety of processes including stone washing of clothing, detergent processes, deinking and biobleaching of paper & pulp and treatment of waste streams.
A neutral and/or alkaline cellulase comprise obtained by a method which comprises:
A composition for the enzymatic treatment of cellulosic fibers or fabrics, comprising a cellulase whose amino acid sequence is encoded by a nucleic acid sequence from a wild type fungus of the genus Chrysoporium, said compostion having a pH between about 8.0 and about 12.0.
A composition for the enzymatic treatment of cellulosic fibers of fabrics, comprising a neutral and/or alkaline cellulose whose amino acid sequence is encoded by a nucleic acid sequence from a wild-type fungus of thegenus Chrysosporium, said composition further comprising one or more components that enhance the performance of the cellulase, wherein the components are selected from the group consisting of added proteases, detergents, and surfactants.
A composition for the enzymatic treatment of cellulosic fibers or fabrics, having from about 33 to 191 units of endo-1,4-.beta.-glucanase activity per gram of dry composition, as measured by an endoviscometric assay, of a neutral and/or alkaline cellulose whose amino acid sequence is encoded by a nucleic acid sequence from a wild-type fungus of the genus Chrysosporium.
A composition for the enzymatic treatment of cellulosic fibers or fabrics, having from about 33 to 191 units of endo-1,4-.beta.-glucanase activity per gram of dry composition, as measured by an endoviscometric assay, of a neutral and/or alkaline cellulose isolated or obtained from a wild-type fungus of the genus Chrysosporium.
A composition for the enzymatic treatment of cellulosic fibers or fabrics, having about 120 to 191 units of endo-1,4-.beta.-glucanase activity per gram of dry composition, as measured by an endoviscometric assay, of a neutral and/or alkaline cellulose whose amino acid sequence is encoded by a nucleic acid sequence from a wild-type fungus of the genus Chrysosporium.
A composition for the enzymatic treatment of cellulose fibers or fabrics, having about 120 to 191 units of endo-1,4-.beta.-glucanase activity per gram of dry composition, as measured by an endoviscometric assay, of a neutral and/or alkaline cellulose isolated or obtained from a wild-type fungus of the genus Chrysosporium.
A laundry detergent composition, comprising a cellulase whose amino acid sequence is encoded by a nucleic aid sequence from a wild-type fungus of the genus Chrysosporium, further comprising a surfactant.
A laundry detergent composition, comprising a cellulase isolated or obtained from a wild-type fungus of the genus Chrysosporium, further comprising a surfactant.
A neutral/alkaline cellulase composition obtained from a wild-type fungus of the species Chrysosporium luckowense.
A neutral/alkaline cellulase composition obtained from a wild-type fungus of the species Chrysosporium lucknowense Garg 27K, accession number VKM F-3500D.
A neutral/alkaline cellulase composition obtained from a wild-type fungus of the genus Chrysosporium, with the proviso that the fungus is not Chrysosporium pruinosum.
A composition for the enzymatic treatment of cellulosic fibers or fabrics, having from 314 to 1537 units of endo-1,4-.beta.-glucanase activity per gram of protein, as measured by an endoviscometric assay, of a neutral and/or alkaline cellulose whose amino acid sequence is encodes by a nucleic acid sequence from a wild-type fungus of the genus Chrysosporium.
A composition for the enzymatic treatment of cellulosic fibers or fabrics, having from 314 to 1537 units of endo-1,4-.beta.-glucanase activity per gram of protein, as measured by an endoviscometric assay, of a neutral and/or alkaline cellulose isolated or obtained from a wild-type fungus of the genus Chrysosporium.
Dependencies are inferred from the claim text.