Methods for the activation of proliferation of animal cells
OwnerWolfgang A. Renner, Hans M. Eppenberger +1inventors
InventorsWolfgang A. Renner, Hans M. Eppenberger, James E. Bailey
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Abstract
Cell cultures are described which proliferate in a serum- and protein-free environment and which are characterized by the circumstance that the concentration of at least one cell-cycle-regulatory protein is increased during at least one period of the cell-cycle. Methods for the production of such cell cultures are presented. With these methods cell lines can be transferred to serum- and protein-free growth that are already in use in a production process which, prior to the use of these methods, requires serum or proteins added to the basal medium.
Claims28
An animal cell which is transfected with an exogenous nucleic acid sequence encoding a cyclin E protein or an E2F transcription factor and which expresses said protein at a level which allows the cell to proliferate in serum-free and protein-free culture media.
A mammalian cell which is transfected with an exogenous nucleic acid sequence encoding a cyclin E protein or an E2F transcription factor and which expresses said protein at a level which allows the cell to proliferate in serum-free and protein-free culture media.
An animal cell which is transfected with an exogenous nucleic acid sequence encoding a cyclin E protein or an E2F transcription factor and which expresses said protein at a level which allows the cell to proliferate in serum-free and protein-free culture media, said cell produced according to the method wherein said exogenous nucleic acid sequence is introduced into an animal cell which grows in a serum-containing and protein-containing environment, the serum-containing and protein-containing environment being replaced by an at least serum-free environment before, at the time of, or after the introduction of said exogenous nucleic acid sequence.
A mammalian cell which is transfected with an exogenous nucleic acid sequence encoding a cyclin E protein or an E2F transcription factor and which expresses said protein at a level which allows the cell to proliferate in serum-free and protein-free culture media, said cell produced according to the method wherein said exogenous nucleic acid sequence is introduced into a mammalian cell which grows in a serum-containing and protein-containing environment, the serum-containing and protein-containing environment being replaced by an at least serum-free environment before, at the time of, or after the introduction of said exogenous nucleic acid sequence.
Dependencies are inferred from the claim text.