Enzymatic DNA molecules
AssigneeThe Scripps Research Institute
InventorsGerald F. Joyce, Ronald R. Breaker
Life of this patent
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Abstract
The present invention discloses deoxyribonucleic acid enzymes--catalytic or enzymatic DNA molecules--capable of cleaving nucleic acid sequences or molecules, particularly RNA, in a site-specific manner, as well as compositions including same. Methods of making and using the disclosed enzymes and compositions are also disclosed.
Claims42
A catalytic DNA molecule having site-specific endonuclease activity, wherein said catalytic DNA molecule comprises a nucleotide sequence selected from the group consisting of:
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A catalytic DNA molecule having site-specific endonuclease activity, wherein said catalytic DNA molecule comprises a nucleotide sequence selected from the group consisting of:
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A catalytic DNA molecule that specifically cleaves a substrate nucleic acid at a defined cleavage site, said catalytic DNA molecule produced by a method comprising the steps of:
- a. admixing a population of single-stranded DNA molecules with ribonucleotide-containing substrate nucleic acid molecules to form an admixture;
- b. maintaining said admixture for a sufficient period of time and under predetermined reaction conditions to allow single-stranded DNA molecules in said population to cause cleavage of said substrate nucleic acid molecules, thereby producing substrate cleavage products;
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- c. isolating single-stranded PND molecules that cleave said substrate nucleic acid molecules from said population.
A method of selecting a catalytic DNA molecule that cleaves a substrate nucleic acid at a specific site, comprising the steps of:
- a. admixing a population of single-stranded DNA molecules with ribonucleotide-containing substrate nucleic acid molecules, to form an admixture;
- b. maintaining said admixture for a sufficient period of time and under predetermined reaction conditions to allow single-stranded DNA molecules in said population to cause cleavage of said substrate nucleic acid molecules, thereby producing substrate cleavage products;
- and
- c. isolating single-stranded DNA molecules that cleave said substrate nucleic acid molecules from said population.
An enzymatic DNA molecule comprising a deoxyribonucleotide polymer having a catalytic activity for cleaving a nucleic acid-containing substrate to produce a cleavage product said enzymatic DNA molecule produced by a method comprising the steps of:
- a. admixing a population of single-stranded DNA molecules with ribonucleotide-containing substrate nucleic acid molecules to form an admixture;
- b. maintaining said admixture for a sufficient period of time and under predetermined reaction conditions to allow single-stranded DNA molecules in said population to cause cleavage of said substrate nucleic acid molecules, thereby producing substrate cleavage products;
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- c. isolating single-stranded DNA molecules that cleave said substrate nucleic acid molecules from said population.
An enzymatic DNA molecule comprising a conserved domain flanked by two substrate binding domains, said enzymatic DNA molecule produced by a method comprising the steps of:
- a. admixing a population of single-stranded DNA molecules with ribonucleotide-containing substrate nucleic acid molecules, to form an admixture;
- b. maintaining said admixture for a sufficient period of time and under predetermined reaction conditions to allow single-stranded DNA molecules in said population to-cause cleavage of said substrate nucleic acid molecules, thereby producing substrate cleavage products;
- and
- c. isolating single-stranded DNA molecules that cleave said substrate nucleic acid molecules from said population.
Dependencies are inferred from the claim text.