Deprotection of RNA with alkylamine
AssigneeRibozyme Pharmaceuticals, Inc.
InventorsNassim Usman, Francine E. Wincott, Danuta Tracz
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Abstract
A method for the deprotection of RNA comprising reacting an alkylamine or ammonium hydroxide/alkylamine mixture with the RNA at 60-70 degrees Centigrade for 5 to 15 minutes in order to remove any exocyclic protecting groups, wherein said alkylamine is selected from the group consisting of ethylamine, propylamine, and butylamine.
Claims1
A method for the deprotection of RNA comprising reacting an alkylamine or ammonium hydroxide/alkylamine mixture with the RNA at 60-70 degrees Centigrade for 5 to 15 minutes in order to remove any exocyclic protecting groups, wherein said alkylamine is selected from the group consisting of ethylamine, propylamine, and butylamine.
Dependencies are inferred from the claim text.