Enzyme-immobilized electrode, composition for preparation of the same and electrically conductive enzyme
AssigneesDai Nippon Printing Co., Ltd., Isao Karube
InventorsIsao Karube, Susan Anne Clark, Ryohei Nagata
Life of this patent
Estimating the term…
Abstract
An enzyme sensor system capable of quantitatively determining a particular component contained in a biological sample in a rapid and easy manner is disclosed. More particularly, an electrically conductive enzyme, an enzyme-immobilized electrode using the same and a composition for use in the preparation of the enzyme electrode are disclosed. The composition for an enzyme-immobilized electrode according to the present invention enables electrodes of various patterns to be produced by screen printing.
Claims7
A process for producing an electrically conductive enzyme comprising an enzyme and a mediator attached to a side chain and the body of the enzyme through a covalent bond wherein said enzyme is glucose oxidase and said mediator is a ferrocene derivative attached to the side chain of the enzyme through a spacer, comprising the steps of
- oxidizing a glucose oxidase with an oxidizing agent to form an aldehyde group on an oligosaccharide of the glucose oxidase;
- reacting said glucose oxidase with a dihydrazide represented by the following formula (II):
- ##STR5## wherein n is an integer of 1 to 7;
- and reacting the reaction product with a ferrocenecarboxylic acid.
A process for preparing an enzyme-immobilized electrode which comprises immobilizing a composition on a base material, the composition comprising an electrically conductive enzyme, an electrically conductive component and a vehicle.
Dependencies are inferred from the claim text.