An air-fuel ratio control system in an internal combustion engine, comprising a fuel injection valve provided at each of plural cylinders, a target air-fuel ratio setting means for setting a target air-fuel ratio among a plurality of predetermined air-fuel ratios including a lower one and a higher one, based on an operational state of the internal combustion engine, and a fuel injection amount control means for separately changing an amount of fuel injected by each said fuel injection valve based on a change in the target air-fuel ratio, in which when said target air-fuel ratio setting means has switched the target air-fuel ratio between said lower one and said higher one, said fuel injection amount control means sequentially and separately changes said amounts of fuel injected by the respective fuel injection valves from an amount suitable for one of said lower and higher air-fuel ratios to another amount suitable for the other of the lower and higher air-fuel ratios, with predetermined time intervals being provided for the changing operations for the fuel injection valves, said fuel injection amount control means including means for changing said time intervals.