Method and composition for inhibiting cellular irreversible changes due to stress
AssigneeGalileo Laboratories, Inc.
InventorsGuy Miller, Lillian Lou, John Nakamura
Life of this patent
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Abstract
Purine derivatives are provided for treatment of cellular stress, particularly hypoxia. By administering the purine derivatives by themselves or in conjunction with other compounds, particularly electron acceptor compounds and/or amino acids, the time for irreversible cellular changes, particularly mortality, can be greatly extended.
Claims20
A method for delaying the time that mammalian cells under stress due to energetic dysfunction undergo irreversible change related to said stress, said method comprising:
- adding to a medium comprising said cells in an amount sufficient to delay the time for said irreversible change at least one of an electron acceptor and an amino acid and a purine derivative other than adenosine triphosphate or adenosine.
A method of inhibiting the effects of hypoxia in a cell or tissue culture, said method comprising:
- adding to said culture in an amount sufficient to delay the time for cells in said cell culture to undergo irreversible changes related to said hypoxia an N.sup.9 -ribosylated purine.
A method of inhibiting the effects of hypoxia in a mammalian host, said method comprising:
- adding to said host in an amount sufficient to delay the time for cells in said host to undergo irreversible changes related to said hypoxia an N.sup.9 -ribosylated purine other than adenosine.
A composition comprising a concentrate capable of forming a solution of a purine derivative in combination with at least one of an electron acceptor and an amino acid, wherein said solution comprises said purine derivative at a concentration in the range of about 0.1 .mu.M to 5 mM and said electron acceptor and/or amino acid are in a molar ratio of 1:1-100 to said purine derivative.
A cellular composition comprising dispersed cells, tissue or viable organ and in an amount sufficient to delay the time for occurrence of an irreversible change in said cells, tissue or viable organ leading to mortality, a purine derivative other than a triphosphate.
In a method for transplanting cells or an organ, where said cells or organ are maintained for a period of time between isolation and implantation, the improvement which comprises:
- storing said cells or organ during said period of time in a composition comprising an N.sup.9 -ribosylated purine.
A composition comprising a concentrate capable of forming a solution of a purine derivative in combination with at least one of an electron acceptor and an amino acid, wherein said solution comprises said purine derivative at a concentration in the range of about 0.1 .mu.M to 5 mM and said electron acceptor and/or amino acid are in a molar ratio of 1:1-100 to said purine derivative, wherein the purine derivative is guanosine.
A cellular composition comprising dispersed cells, tissue or viable organ and in an amount sufficient to delay the time for occurrence of an irreversible change in said cells, tissue or viable organ leading to mortality, a purine derivative other than a triphosphate or adenosine.
In a method for transplanting cells or an organ, where said cells or organ are maintained for a period of time between isolation and implantation, the improvement which comprises:
- storing said cells or organ during said period of time in a composition comprising guanosine.
Dependencies are inferred from the claim text.