DNA encoding threonyl tRNA synthetase from staphylococcus aureus
AssigneeSmithKline Beecham Corporation
InventorsJohn Edward Hodgson, Elizabeth Jane Lawlor
Life of this patent
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Abstract
The invention provides tRNA synthetase polypeptides and DNA (RNA) encoding tRNA synthetase polypetides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing tRNA synthetase polypeptide for the protection against infection, particularly bacterial infections.
Claims24
An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of:
- (a) a polynucleotide having at least a 95% identity to a polynucleotide encoding a polypeptide comprising amino acids 1 to 645 of SEQ ID NO:2;
- and
- (b) a polynucleotide which is complementary to the polynucleotide of (a).
An isolated polynucleotide comprising a member selected from the group consisting of:
- (a) a polynucleotide having at least a 95% identity to a polynucleotide encoding the same mature polypeptide expressed by the threonyl tRNA synthetase gene contained in NCIMB Deposit No. 40771;
- and
- (b) a polynucleotide complementary to the polynucleotide of (a).
An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of:
- (a) a polynucleotide having at least a 97% identity to a polynucleotide encoding a polypeptide comprising amino acids 1 to 645 of SEQ ID NO:2;
- and
- (b) a polynucleotide which is complementary to the polynucleotide of (a).
An isolated polynucleotide comprising a member selected from the group consisting of:
- (a) a polynucleotide having at least a 97% identity to a polynucleotide encoding the same mature polypeptide expressed by the threonyl tRNA synthetase gene contained in NCIMB Deposit No. 40771;
- and
- (b) a polynucleotide complementary to the polynucleotide of (a).
An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of:
- (a) a polynucleotide hybridizing under stringent conditions to a polynucleotide encoding a polypeptide comprising amino acids 1 to 645 of SEQ ID NO:2;
- and
- (b) a polynucleotide which is complementary to the polynucleotide of (a).
An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of:
- (a) a polynucleotide encoding a polypeptide comprising amino acids 1 to 645 of SEQ ID NO:2;
- and
- (b) a polynucleotide which is complementary to the polynucleotide of (a).
The isolated polynucleotide of SEQ ID NO:1.
An isolated polynucleotide comprising a DNA sequence obtained by screening an appropriate library containing the complete gene encoding an amino acid sequence set forth in SEQ ID NO:2 under stringent hybridization conditions with a probe having a polynucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:2 or a fragment thereof, which fragment retains binding and/or catalytic activity;
- and isolating said DNA sequence.
An isolated polynucleotide comprising nucleotides 1 to 1935 set forth in SEQ ID NO:1.
Dependencies are inferred from the claim text.