Oil-body protein cis-elements as regulatory signals
AssigneeSemBioSys Genetics Inc.
InventorMaurice M. Moloney
Life of this patent
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Abstract
DNA constructs comprising 5' untranslated sequences from genes active from the late globular stage through to embryo maturity are provided. These constructs may be used to obtain expression of a DNA sequence of interest during phases of embryogenesis which precede the accumulation of storage proteins.
Claims37
A method of expressing a DNA sequence of interest in a seed cell, said method comprising:
- growing a plant capable of developing seed, wherein said plant comprises cells containing integrated into their genome an expression cassette comprising in the 5'-3' direction of transcription as operably linked components, a transcriptional regulatory region obtained from an oil body protein gene, a DNA sequence of interest heterologous to said regulatory region, and a transcriptional termination region, under conditions whereby seed is produced in which said DNA sequence is expressed under transcriptional control of said regulatory region and during a phase of embryogensesis which precedes accumulation of storage proteins.
A DNA construct comprising:
- a chimeric gene comprising a transcriptional regulatory region obtained from an Arabidopsis oil body protein gene fused to a DNA sequence of interest heterologous to said regulatory region.
An expression casette comparing:
- as operably linked components, a transcriptional regulatory region obtained from Arabidopsis oil body protein gene, a DNA sequence of interest heterologous to said regulatory region, and a transcriptional termination region.
An isolated transcriptional regulatory region comprising a DNA molecule comprising the sequence shown in SEQ.ID.NO:1 from nucleotide 1 to nucleotide 867.
A plant comprising:
- cells containing integrated into their genome, a chimeric gene comprising a transcriptional regulatory region obtained from an Arabidopisis oil body protein gene, wherein said transcriptional regulatory region is fused to a DNA sequence of interest heterologous to said regulatory region.
Seed comprising:
- cells containing integrated into their genome, a chimeric gene comprising a transcriptional regulatory region obtainable from an Arabidopsis oil body protein gene, wherein said transcriptional regulatory region is fused to a DNA sequence of interest heterologous to said regulatory region.
A method for altering seed-specific metabolism, said method comprising:
- growing a plant capable of developing seed, wherein said plant comprises cells containing integrated into their genome an expression cassette comprising in the 5'-3' direction of transcription, a transcriptional initiation region obtained from an oil body protein gene, a DNA sequence of interest other than a sequence native to said initiation region, and a transcriptional termination region, under conditions whereby seed is produced in which said DNA sequence is expressed under transcriptional control of said transcriptional initiation region wherein expression of said DNA sequence altars seed-specific metabolism during a phase of embroyogenesis which precedes accumulation of storage proteins.
A method for producing heterologous polypeptide in seed, said method comprising:
- growing a plant capable of developing seed, wherein said plant comprises cells containing integrated into their genome an expression cassette comprising in the 5'-3' direction of transcription, a transcriptional initiation region obtained from an oil body protein gene, a DNA sequence of interest encoding a polypeptide heterologous to said plant, and a transcriptional termination region, under conditions whereby seed is produced in which said DNA sequence is expressed under transcriptional control of said transcriptional initiation region and during a phase of embryogenesis which precedes accumulation of storage proteins.
An isolated transcriptional regulatory region obtained from an Arabidopsis oil body protein gene.
Dependencies are inferred from the claim text.