Nucleotides encoding angiostatin protein and method of use
AssigneeChildren's Medical Center Corporation
InventorsMichael S. O'Reilly, M. Judah Folkman
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Abstract
The present invention comprises an endothelial inhibitor and method of use therefor. The endothelial inhibitor is a protein isolated from the blood or urine that is eluted as a single peak from C4-reverse phase high performance liquid chromatography. The endothelial inhibitor is a molecule comprising a protein having a molecular weight of between approximately 38 kilodaltons and 45 kilodaltons as determined by reducing polyacrylamide gel electrophoresis and having an amino acid sequence substantially similar to that of a murine plasminogen fragment beginning at amino acid number 98 of a murine plasminogen molecule.
Claims26
A composition comprising, an isolated nucleotide molecule having a sequence that codes for angiostatin protein containing approximately kringle region 1 through 4 of a plasminocen molecule, the angiostatin protein having anti-angiogenic activity, and having a molecular weight of between approximately 38 and 45 kilodaltons as determined by reducing polyacrylamide gel electrophoresis.
A composition comprising, an isolated nucleotide molecule having a sequence that codes for angiostatin protein having anti-angiogenic activity, and wherein the anziostatin protein contains approximately kringle regions 1 through 4 of a plasminogen molecule.
A composition comprising, an isolated nucleotide molecule having a sequence that codes for angiostatin protein having a molecular weight of between approximately 38 and 45 kilodaltons as determined by reducing polyacrylamide gel electrophoresis, having anti-angiogenic activity, and having an amino acid sequence substantially similar to a plasminogen fragment beginning at about amino acid 98 of a plasminogen molecule.
Dependencies are inferred from the claim text.