Zinc finger proteins with high affinity new DNA binding specificities
AssigneeMassachusetts Institute of Technology
InventorsEdward J. Rebar, Carl O. Pabo
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Abstract
Described is a polypeptide comprising one or more zinc fingers. The polypeptide binds to new polynucleotide subsites with high affinity and consequently has a binding specificity that differs from wild type zinc finger proteins. The binding occurs through contacts between certain amino acid residues of the zinc fingers and the nucleic acids of the subsites. The polypeptide sequence of at least one zinc finger differs from wild type zinc fingers, and the difference involves at least one amino acid residue that contacts the bases of the polynucleotide during binding.
Claims8
A polypeptide comprising three zinc fingers, each of said zinc fingers comprising a set of base-contacting amino acid residues, wherein:
- a) at least one set of base-contacting amino acid residues in at least one zinc finger differs in sequence at one or more base-contacting amino acid residues as compared with a corresponding set of base-contacting amino acid residues in any known wild type zinc finger protein;
- b) the polypeptide has a DNA base sequence specificity different from that of any known wild type zinc finger protein;
- and
- c) the polypeptide binds a targeted polynucleotide with a dissociation constant of less than about 1.0 nanomolar.
A polypeptide comprising three zinc fingers, wherein the polypeptide
- a) has essentially the same amino acid sequence as a wild type zinc finger protein, further characterized by at least one or more point mutations at one or more base-contacting amino acid residues;
- b) has a base sequence specificity different from the wild type zinc finger protein;
- and
- c) binds a targeted polynucleotide with a dissociation constant of less than about 1.0 nanomolar.
Dependencies are inferred from the claim text.