Alphavirus structural protein expression cassettes
AssigneeChiron Corporation
InventorsThomas W. Dubensky, Jr., John M. Polo, Carlos E. Ibanez, Stephen M. W. Chang, Douglas J. Jolly, David A. Driver
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Abstract
The present invention provides compositions and methods for utilizing recombinant alphavirus vectors. Also disclosed are compositions and methods for making and utilizing eukaryotic layered vector initiation systems.
Claims29
A DNA alphavirus structural protein expression cassette, comprising an inducible promoter and an alphavirus structural protein gene, wherein the promoter directs the expression of the alphavirus structural protein gene upon induction of the promoter within a cell, and wherein prior to induction within the cell, the expression cassette does not express sufficient quantities of structural proteins to be cytotoxic to a BHK cell containing the expression cassette.
A DNA alphavirus structural protein expression cassette, comprising a promoter 5' of viral cDNA which initiates the synthesis of RNA from the viral cDNA within a cell, followed by a 5' sequence which initiates transcription of alphavirus RNA from viral RNA, a viral junction region promoter operably-linked to one or more alphavirus structural protein genes and an alphavirus RNA polymerase recognition sequence, with the proviso that the cassette does not direct the expression of all alphavirus nonstructural protein genes.
An alphavirus structural protein expression cassette, comprising a promoter and an alphavirus glycoprotein gene, wherein the promoter directs the expression of the alphavirus glycoprotein gene, with the proviso that the promoter does not direct the expression of an alphavirus capsid protein gene.
An alphavirus structural protein expression cassette, comprising a promoter and an alphavirus structural protein gene, wherein the promoter directs the expression of the alphavirus structural protein gene, with the proviso that the promoter does not direct the expression of an alphavirus glycoprotein gene, nor the expression of all alphavirus nonstructural protein genes.
An alphavirus structural protein expression cassette, comprising a promoter, an alphavirus structural protein gene, and a heterologous ligand sequence, wherein the promoter directs the expression of the alphavirus structural protein gene and the heterologous ligand sequence, with the proviso that the promoter does not direct the expression of all alphavirus nonstructural protein genes.
Dependencies are inferred from the claim text.