Glycorproteins, antibodies, and diagnostic kits for detection of bovine herpesvirus type 1
AssigneeStichting Centraal Diergeneeskundig Instituut
InventorsFranciscus Antonius Maria Rijsewijk, Johannes Theodorus van Oirschot, Roger Kamiel Maes
Life of this patent
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Abstract
Deletion mutant of bovine herpesvirus type 1 which has a deletion in the glycoprotein gE-gene. The mutant may further have a deletion in the thymidine kinase gene and/or the glycoprotein gI-gene, or have an insertion of a heterologous gene. Recombinant nucleic acid which comprises the gE-gene or a part thereof. Glycoprotein gE, peptides based thereon and complexes of the glycoproteins gE and gI, and antibodies against them. Vaccines and diagnostic kits comprising any one of these materials.
Claims32
A glycoprotein composition comprising glycoprotein E (gE) of bovine herpesvirus type 1 (BHV-1) or a complex of the BHV-1 glycoproteins gE and gI, in a suitable buffer.
An antibody composition comprising an antibody specific for glycoprotein E (gE) of bovine herpesvirus type 1 (BHV-1) or a complex of the BHV-1 glycoproteins gE and gI, in a suitable buffer.
A diagnostic kit for detecting antibodies specific for bovine herpesvirus type 1 (BHV-1) in a sample from an animal comprising glycoprotein E (gE) of BHV-1 or a complex of the BHV-1 glycoproteins gE and gI, and a detection means suitable for an antibody detection assay.
A diagnostic kit for a blocking immunoassay to detect antibodies specific for bovine herpesvirus type 1 (BHV-1), in a sample from an animal, comprising an antibody specific for glycoprotein E (gE) of BHV-1, or a complex of the BHV-1 glycoproteins gE and gI, and a detection means suitable for an antibody detection assay.
A diagnostic kit for detecting bovine herpesvirus type 1 (BHV-1) in a sample comprising an antibody which is specific for glycoprotein E (gE) of BHV-1, or a complex of the BHV-1 glycoproteins gE and gI, and a detection means suitable for BHV-1 detection assay.
A method of determining bovine herpesvirus type 1 (BHV-1) infection of an animal, comprising examining a sample from an animal for the presence of (A) gE of BHV-1 or a complex of the BHV-1 glycoproteins gE and gI, or (B) antibodies specific for gE of BHV-1 or a complex of the BHV-1 glycoproteins gE and gI, by the steps of:
- providing a support having thereon (C) antibodies specific for gE of BHV-1 or said complex of the BHV-1 glycoproteins gE and gI, or (D) gE of BHV-1 or a complex of the BHV-1 glycoproteins gE and gI;
- adding said sample to said support;
- adding a chromogenic substrate to said support;
- and
- detecting the presence of said antibodies, or said gE or said complex of gE and gI.
Dependencies are inferred from the claim text.