Adsorbents for amino acid and peptide separation
AssigneeCalifornia Institute of Technology
InventorsFrances H. Arnold, Vidyasankar Sundaresan
Life of this patent
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Abstract
An adsorbent which selectively binds to one enantiomer of an optically active amino acid or peptide. The adsorbent includes a polymer matrix which contains one or more metal complexes that are oriented within the polymer matrix by molecular imprinting to provide selective binding of the matrix to only one enantiomer of the optically active amino acid or peptide. Separation systems are disclosed which use the adsorbent as the basis for conducting enantioresolution of optically active amino acids and peptides. Methods for using the adsorbent are also disclosed.
Claims23
An adsorbent which selectively binds to a target molecule which is an enantiomer of an optically active amino acid or peptide, said adsorbent comprising:
- an imprinted polymer matrix comprising a porous polymer and a plurality of metal complexes, each of said metal complexes comprising a polymerizable moiety and a ligand moiety, said metal complexes being linked to said porous polymer via said polymerizable moiety during imprinting polymerization in the presence of said target molecule or analog thereof, wherein said imprinted polymer matrix selectively binds to said target molecule;
- and
- a support structure having a surface, said imprinted polymer matrix being attached to said support structure as a coating thereon.
An adsorbent which selectively binds to a target molecule which is an enantiomer of an optically active amino acid or peptide, said adsorbent comprising:
- an imprinted polymer matrix comprising a porous polymer and a plurality of metal complexes, each of said metal complexes comprising a polymerizable moiety and a ligand moiety, said metal complexes being linked to said porous polymer via said polymerizable moiety during imprinting polymerization in the presence of said target molecule or analog thereof, said metal complexes having one of the following structures:
- ##STR2## where M is copper, zinc, cobalt, vanadium, mercury, iron, aluminum, silver, barium, borate, calcium, Y.sup.3+ and La.sup.3+ ;
- S.sub.1 and S.sub.2 are solvent components selected from the group consisting of H.sub.2 O, OH.sup.- and other counter anions from said solution, such as Cl.sup.-, PO4.sup.-3, SO.sub.4.sup.-2 or perchlorate;
- X is a functional group selected from the group consisting of styrene, methacrylate, acrylate, vinyl ether, vinyl acetate, trialkoxysilane, dialkylchlorosilane, epoxy and alkylhydroxy or alkylamine groups having from 1 to 3 carbon atoms;
- L.sub.1 and L.sub.2 are selected from the group consisting of N, COO.sup.- or O.sup.- ;
- and Y and Z is either H or a functional group selected from the group consisting of styrene, methacrylate, acrylate, vinyl ether, vinyl acetate, trialkoxysilane, dialkylchlorosilane, epoxy and alkylhydroxy and alkylamine groups having from 1 to 3 carbon atoms, said metal complexes being linked to said porous polymer via X and Y and/or Z to provide selective binding of said ligand moieties with said target molecule.
Dependencies are inferred from the claim text.