Method for producing improved sensor
AssigneesAustralian Membrane and Biotechnology Research Institute, The University of Sydney
InventorsBurkhard Raguse, Bruce A. Cornell, Vijoleta L. Braach-Maksvytis, Ronald J. Pace
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Abstract
The present invention relates to a novel method for producing an electrode membrane combination. This novel method employs compound including a linker lipid for use in attaching a membrane including a plurality of ionophores to an electrode and providing a space between the membrane and the electrode, the electrode being either in part or totally made up of the linker lipid. The linker lipid comprises within the same molecule a hydrophobic region capable of spanning the membrane, an attachment group used to attach the molecule to an electrode surface, and a hydrophilic region intermediate said hydrophobic region and the attachment group.
Claims20
A method of producing an electrode membrane combination comprising the steps of:
- (1) Forming a solution containing reservoir lipids comprising within a same molecule an attachment region, hydrophobic regions, and an hydrophilic region positioned between the attachment region and the hydrophobic region, and optionally a head group attached to the hydrophobic region made from the hydrophilic region;
- and spacer compounds comprising within the same molecule a hydrophilic group and an attachment group;
- (2) contacting the electrode with the solution from step (1), the composition of the electrode and the attachment regions being selected such that the attachment regions chemisorbed to the electrode;
- (3) rinsing the electrode;
- (4) contacting the coated electrode from step (3) with a solution of lipid and ionophore in a carrier solvent containing less than 2% of an alkane;
- and
- (5) adding an aqueous solution to the electrode from step (4).
Dependencies are inferred from the claim text.