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AssigneeHuman Cell Cultures Inc.
InventorsHayden G. Coon, Francesco Saverio Ambesi-Impiombato, Francesco Curcio
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The present invention provides a method for producing an expanded non-transformed cell culture comprising the steps of: (1) preparing partially purified, minced tissue; (2) concentrating the resulting cells and tissue pieces; (3) resuspending the concentrated tissue cells and pieces in a culture medium capable of supporting sustained cell division that is contained in a culture vessel; (4) incubating the cells; and (5) passaging the cells periodically. The present invention further provides clonal strains of cells derived from the above-mentioned cell culture, medium and conditioned medium designed for the culturing of such cells, including pancreatic, thyroid, parathyroid, and parotid cells, and the use of cultured pancreatic cells to form pancreatic pseudotissues composed of matrix-embedded aggregated (pseudoislets) or individual cells, to treat blood sugar disorders in mammals, and to test for cytotoxicity and autoimmune activities with reference to pancreatic endocrine cells.
A method of altering blood sugar levels of a mammal comprising administering expanded human pancreatic cells to the mammal, which expanded cells have been expanded in a cell culture and comprise enriched, non-transformed, serially passaged human pancreatic endocrine cells, wherein said cell culture is substantially free of fibroblast, macrophage, and capillary endothelial cells, and wherein said cell culture is prepared by a method comprising the steps of:
A method of altering blood sugar levels of a human comprising administering expanded pancreatic cells of human origin to the human, which expanded cells have been expanded in a cell culture and comprise enriched, non-transformed, serially passaged pancreatic endocrine cells, wherein said cell culture is substantially free of fibroblast, macrophage, and capillary endothelial cells, and wherein said cell culture is prepared by a method comprising the steps of:
A method of altering the blood sugar of a mammal comprising administering to said mammal non-transformed mammalian cells obtained from a non-transformed cell culture, wherein said cell culture comprises:
Dependencies are inferred from the claim text.