Production of phytate degrading enzymes in trichoderma
AssigneeAlko Group Ltd.
InventorsHelena K. M. Nevalainen, Marja T. Paloheimo, Aria S. K. Miettinen-Oinonen, Tuula K. Torkkeli, Michael Cantrell, Cristopher S. Piddington, John A. Rambosek, Marja K. Turunen, Richard B. Fagerstrom
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Abstract
A highly efficient overexpression system for phytase and pH 2.5 acid phosphatase in Trichoderma is described. This system results in enzyme compositions that are especially useful in the animal feed industry.
Claims13
Culture medium obtained after the culture of transformed Trichoderma host cells, said culture medium comprising an Aspergillus phytate degrading enzyme that has a Trichoderma glycosylation pattern, said culture medium being produced by a process comprising:
- (a) culturing a transformed Trichoderma host cell, wherein said host cell has been transformed with a gene encoding said phytate degrading enzyme, said phytate degrading enzyme being selected from the group consisting of:
- (i) phytase, wherein said gene comprises DNA encoding amino acids 20-467 of SEQ ID NO:8;
- and
- (ii) pH 2.5 acid phosphatase, wherein said gene comprises DNA encoding amino acids 20-479 of SEQ ID NO:2;
- and
- (b) expressing said gene encoding said phytate degrading enzyme in said Trichoderma host cell.
A composition comprising an Aspergillus pH 2.5 acid phosphatase that has a Trichoderma glycosylation pattern, said pH 2.5 acid phosphatase being produced by a process comprising:
- (a) culturing a transformed Trichoderma host cell, wherein said host cell has been transformed with a gene encoding said pH 2.5 acid phosphatase, wherein said gene comprises DNA encoding amino acids 20-479 of SEQ ID NO:2;
- (b) expressing said pH 2.5 acid phosphatase in said Trichoderma host cell.
Dependencies are inferred from the claim text.