Method and reagent for detecting peroxidase or hydrogen peroxide
AssigneesYamagata Technopolis Foundation, JEOL Ltd.
InventorsMasaaki Aoyama, Masanobu Shiga
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Abstract
Method for detecting peroxidase or hydrogen peroxide with high sensitivity. Both peroxidase and hydrogen peroxide are prepared such that one of them is overabundant to the other. Phenoxy radicals are produced from a p-substituted phenol compound by the action of peroxidase in the presence of hydrogen peroxide. The free radicals are trapped by a hydroxy amine compound, and stable radical species are produced. Electron spin resonances of the stable radical species are measured.
Claims8
A method for detecting peroxidase or hydrogen peroxide, said method comprising the steps of:
- preparing peroxidase and hydrogen peroxide such that one of them is overabundant to the other;
- producing phenoxy radicals from a p-substituted phenol compound by the action of the peroxidase in the presence of the hydrogen peroxide;
- transferring electrons from said phenoxy radicals to a hydroxy amine compound and producing stable radical species of said hydroxy amine compound;
- and
- measuring electron spin resonances of said stable radical species.
A reagent for detection of peroxidase or hydrogen peroxide, said reagent consisting of a hydroxy amine compound which can accent electrons transferred from phenoxy radicals to produce stable radical species of said hydroxy amine compound, said phenoxy radicals formed by the action of peroxidase in the presence of hydrogen peroxide and a p-substituted phenol compound.
A hydroxy amine compound capable of accepting electrons transferred from phenoxy radicals created by the action of peroxidase in the presence of hydrogen peroxide and a p-substituted phenol derivative and which can produce stable radical species of said hydroxy amine compound.
Dependencies are inferred from the claim text.