# US 5,776,759 A · Two novel human cathepsin proteins

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- Number: US 5,776,759 A
- Document ID: US5776759A
- Status: Granted
- Kind: A
- Application number: 08/723,938
- Filed: 1996-09-26
- Granted: 1998-07-07
- Earliest claimed: 1996-09-26
- Assignees: Incyte Pharmaceuticals, Inc.
- Inventors: Olga Bandman; Roger Coleman
- Web page: https://patentgrep.com/patent/US5776759A

## CPC

- G01N 33/573: for enzymes or isoenzymes
- A61P 29/00: Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
- A61P 35/00: Antineoplastic agents
- C12N 9/6421: {from mammals}
- C12N 9/6478: {Aspartic endopeptidases (3.4.23)}
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- G01N 2333/96413: Cysteine endopeptidases (3.4.22)
- G01N 2500/02: Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The present invention provides two novel human cathepsin proteins (HCPs) and polynucleotides encoding HCPs. The invention provides for genetically engineered expression vectors and host cells comprising the nucleic acid sequence encoding HCPs. The invention also provides for the production and use of antibodies to HCPs in pharmaceutical compositions for the treatment of disease processes that include cancers, inflammation, metastasis and peptide and proenzyme processing. In addition, the invention provides for the production and use of inhibitors of HSPs in pharmaceutical compositions for the treatment of diseases. The invention also describes diagnostic assays which utilize the polynucleotide to hybridize with the transcripts encoding HCPs. The invention also provides for the use of antisense molecules in pharmaceutical compositions as a therapeutics in cancers, inflammation, metastasis and peptide and proenzyme processing.

## Claims

### Claim 1

An isolated polynucleotide sequence encoding the polypeptide comprising the amino acid sequence of SEO ID NO:1.

### Claim 2

An isolated polynucleotide sequence consisting of the nucleic acid sequence of SEQ ID NO:2.

### Claim 3 (depends on 2)

An expression vector containing the polynucleotide sequence of claim 2.

### Claim 4 (depends on 3)

A host cell containing the expression vector of claim 3.

### Claim 5 (depends on 4)

A method for producing the polypeptide consisting of SEQ ID NO:1, the method comprising the steps of:
a) culturing the host cell of claim 4 under conditions suitable for the expression of the polypeptide; and
b) recovering the polypeptide from the host cell culture.

### Claim 6

A hybridization probe consisting of SEQ ID NO:2.

### Claim 7

An isolated polynucleotide sequence which specifically hybridizes to SEQ ID NO:2.

### Claim 8

An isolated polynucleotide sequence consisting of the complement of SEQ ID NO:2.